Selectivity of fluorescence recovery after photobleaching assays. Half-times of fluorescence recovery (t½) expressed as a percentage of the relevant wild-type control cells without inhibitor. Cells were transfected with the indicated bromodomain target and treated with 1 μM PFI-1. Light bars depict assays without suberoylanilide hydroxamic acid (SAHA) addition, and dark bars depict assays with SAHA addition (2.5 μM). The dotted line demarks the point equivalent to 100% of the relevant wild-type control cells without inhibitor. Error bars depict the standard error of the mean. Only bars marked with an asterisk indicate a significant difference from controls (P < 0.05).