Figure 4

Comparison of T2C with 3C-qPCR for the β-globin promoter. T2C for mouse primary erythroid cells (A) and mouse fetal brain cells (C) from E12.5 mice, revealed the same interactions from the β-globin promoter when comparing them to 3C-qPCR (B). The 3C-qPCR was adapted and modified from Drissen et al.[16] with blue line depicting the interactions for primary erythroid cells and with grey the interactions for mouse fetal brain cells from E12.5 mice. White lines indicate the areas of particular interest (such as 3’HS1, β-globin promoter, Locus Control Region (LCR) and 5′ HS-60/-62) in the β-globin locus. Interactions between LCR, the β-globin promoter and the 3′HS1 are lost in mouse brain cells. The shaded vertical bars indicate the comparison between the different panels. The red vertical bar indicates the β-globin promoter. All the T2C interactions are normalized to the same color code (see color inset). The bottom tracks show a linear representation of the β-globin locus, the oligonucleotides probes positions (black lines), Hin dIII recognition sites (red lines) and the ChIP-seq derived binding sites of PolII (red lines), Ldb1 (purple lines) [38], Ctcf (black lines), p300 (black lines), and various histone modification markers (light blue, dark blue, green, and red) [37] in mouse erythroleukemia cells.