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Figure 2 | Epigenetics & Chromatin

Figure 2

From: Vertebrate GAGA factor associated insulator elements demarcate homeotic genes in the HOX clusters

Figure 2

Intergenic HFRs function as enhancer blockers. The human erythroleukemic cell line K562 was stably transfected with different constructs carrying test fragments from the Hox clusters, including intergenic H3-free target regions (dark blue bars) and H3-enriched genic controls (red bars). The inset shows the map of the vector used for the boundary assay. Empty vector negative control and two known boundary constructs (positive controls) carrying the chicken β-globin boundary (control boundary) and the previously characterized Hoxd13-Evx2 intergenic boundary (HoxD boundary) are indicated with light blue bars. The ratio of survival of average number of colonies with each construct normalized to the empty vector is shown (%) on the y axis. Dotted lines indicate levels of boundary activity observed based on the positive and negative controls, with the unfilled star marking the region of negligible activity comparable to negative control and filled stars indicating regions encompassing increasingly efficient enhancer-blocking activity comparable to the positive controls. Results shown are the mean ± standard error from two independent biological replicate experiments, each performed in triplicate.

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