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Table 1 Karyotype analysis for H3f3b wildtype (WT) and knockout (KO) mouse embryonic fibroblasts (MEFs)

From: Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development

  Total spreads Normal spreads Normal ≥ 4N >2 defects Chromosome fragments or breaks Chromatid breaks Triradial Endo-reduplication Rearrangements
WT1 35 26 5 0 2 0 0 0 0
WT2 35 20 10 2 0 4 0 0 0
Total WT 70 46 15 2 2 4 0 0 0
% Total   65.7% 21.4% 2.9% 2.9% 5.7% 0% 0% 0%
KO1 34 16 6 5 4 4 1 1 1
KO2 33 16 6 3 6 6 2 1 2
Total KO 67 32 12 12 10 10 3 2 3
% Total   47.8% 17.9% 17.9% 14.9% 14.9% 4% 3% 4%
  1. Karyotype analysis done on E12.5 WT (H3f3bWT/WT) and KO (H3f3bΔ/Δ) MEFs (lines WT1, WT2 and KO1, KO2 respectively). Normal spreads included typical metaphase spread preparation artifacts. Typical artifacts were the loss of 1 to 2 chromosomes per spread. KO MEFs exhibited a greater incidence of abnormalities.