Table 2 Macronucleus specific primers for bisulfite analysis
Position | Primer | Sequence |
|---|---|---|
1 | Actin 1 for | 5′-GAGAGTATTAGATGTATTGATTAGG-3′ |
1 | Actin 1 rev | 5′-AATTTAAATCATCTTCTCTCTATTAATC-3′ |
2 | Actin 2 for | 5′-GATTAATAGAGAGAAGATGATTTAAATT-3′ |
2 | Actin 2 rev | 5′-AAAATCTCTTCTAACATCAACATC-3 |
3 | Actin 3 for | 5′-GATGTTGATGTTAGAAGAGATTTT-3′ |
3 | Actin 3 rev | 5′-CCCCAAAACCCCATTTAATAA-3′ |
1 | Tubulin 1 for | 5′-TTTGAATATAAGGATATTAATTTTAAGAA-3′ |
1 | Tubulin 1 rev | 5′-ATTCCCATACCTAAACCAATAC-3′ |
2 | Tubulin 2 for | 5′-GTATTGGTTTAGGTATGGGAAT-3′ |
2 | Tubulin 2 rev | 5′-ATAAATTATTCACCAACTCTCTTA-3′ |
1 | Histone 1 for | 5′-GTTTAAAGGATTTGAAAGGAAAAAAGATT-3′ |
1 | Histone 1 rev | 5′-CCCTAAAAACTTCATAATATCTCC-3′ |
2 | Histone 2 for | 5′-GGAGATATTATGAAGTTTTTAGGG-3′ |
2 | Histone 2 rev | 5′-AACATATCAAAAACTTCATCCTC-3′ |
1 | Mdp1 1 for | 5′-TCCAACTTCTCCTTTATCTTCTCTTTCACA-3′ |
1 | Mdp1 1 rev | 5′-GTAGTTTYAGAATTYGAYAGGTAAATTAAATA-3′ |
2 | Mdp1 2 for | 5′-GGGATTAGTATTAATAGTGTTTTTTAAG-3′ |
2 | Mdp1 2 rev | 5′-AAATCTCAATCCTCTCATAAATAAATTA-3′ |
3 | Mdp1 3 for | 5′-TAATTTATTTATGAGAGGATTGAGATTT-3′ |
3 | Mdp1 3 rev | 5′-AAACTCATTAAAAAATCAACTCTCTC-3′ |
4 | Mdp1 4 for | 5′-GAGAGAGTTGATTTTTTAATGAGTTT-3′ |
4 | Mdp1 4 rev | 5′-ATCATAAATAAAATCTTCCCTTTC-3′ |
5 | Mdp1 5 for | 5′-GAAAGGGAAGATTTTATTTTATGAT-3′ |
5 | Mdp1 5 rev | 5′-GAGATGTGGGATCTTTCAAAAACCC-3′ |
1 | Mdp2 1 for | 5′-AAGGAGAGTATAATATGATTGGAYTGTTGA-3′ |
1 | Mdp2 1 rev | 5′-AATCAATATTCCTAACATATCTTCTC-3′ |
2 | Mdp2 2 for | 5′-GAGAAGATATGTTAGGAATATTGATT-3′ |
2 | Mdp2 2 rev | 5′-ATCCTCCTCTTCTCATACC-3′ |