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Table 2 Macronucleus specific primers for bisulfite analysis

From: A permissive chromatin structure is adopted prior to site-specific DNA demethylation of developmentally expressed genes involved in macronuclear differentiation

Position Primer Sequence
1 Actin 1 for 5′-GAGAGTATTAGATGTATTGATTAGG-3′
1 Actin 1 rev 5′-AATTTAAATCATCTTCTCTCTATTAATC-3′
2 Actin 2 for 5′-GATTAATAGAGAGAAGATGATTTAAATT-3′
2 Actin 2 rev 5′-AAAATCTCTTCTAACATCAACATC-3
3 Actin 3 for 5′-GATGTTGATGTTAGAAGAGATTTT-3′
3 Actin 3 rev 5′-CCCCAAAACCCCATTTAATAA-3′
1 Tubulin 1 for 5′-TTTGAATATAAGGATATTAATTTTAAGAA-3′
1 Tubulin 1 rev 5′-ATTCCCATACCTAAACCAATAC-3′
2 Tubulin 2 for 5′-GTATTGGTTTAGGTATGGGAAT-3′
2 Tubulin 2 rev 5′-ATAAATTATTCACCAACTCTCTTA-3′
1 Histone 1 for 5′-GTTTAAAGGATTTGAAAGGAAAAAAGATT-3′
1 Histone 1 rev 5′-CCCTAAAAACTTCATAATATCTCC-3′
2 Histone 2 for 5′-GGAGATATTATGAAGTTTTTAGGG-3′
2 Histone 2 rev 5′-AACATATCAAAAACTTCATCCTC-3′
1 Mdp1 1 for 5′-TCCAACTTCTCCTTTATCTTCTCTTTCACA-3′
1 Mdp1 1 rev 5′-GTAGTTTYAGAATTYGAYAGGTAAATTAAATA-3′
2 Mdp1 2 for 5′-GGGATTAGTATTAATAGTGTTTTTTAAG-3′
2 Mdp1 2 rev 5′-AAATCTCAATCCTCTCATAAATAAATTA-3′
3 Mdp1 3 for 5′-TAATTTATTTATGAGAGGATTGAGATTT-3′
3 Mdp1 3 rev 5′-AAACTCATTAAAAAATCAACTCTCTC-3′
4 Mdp1 4 for 5′-GAGAGAGTTGATTTTTTAATGAGTTT-3′
4 Mdp1 4 rev 5′-ATCATAAATAAAATCTTCCCTTTC-3′
5 Mdp1 5 for 5′-GAAAGGGAAGATTTTATTTTATGAT-3′
5 Mdp1 5 rev 5′-GAGATGTGGGATCTTTCAAAAACCC-3′
1 Mdp2 1 for 5′-AAGGAGAGTATAATATGATTGGAYTGTTGA-3′
1 Mdp2 1 rev 5′-AATCAATATTCCTAACATATCTTCTC-3′
2 Mdp2 2 for 5′-GAGAAGATATGTTAGGAATATTGATT-3′
2 Mdp2 2 rev 5′-ATCCTCCTCTTCTCATACC-3′