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Table 1 Macronucleus specific primers for the N-ChIP analyses

From: A permissive chromatin structure is adopted prior to site-specific DNA demethylation of developmentally expressed genes involved in macronuclear differentiation

Position Primer Sequence
mdp1 5′ Piwi a 5′-CCGTAGTTTCAGAATTCGACAGG-3′
mdp1 5′ Piwi b 5′-GTTGAGGCCTCGACAACTTAAAA-3′
mdp1 3′ Piwi3′-UTR 5′-GTAGGGTCTCTCATCTCCTGTTCGC-3′
mdp1 3′ Piwif 5′-CTTGTCTGGTGTATCACCGATACCATC-3′
mdp2 5′ mdp2-1a 5′- CTTGTCTGGTGTATCACCGATACCATC- 3′
mdp2 5′ mdp2-b 5′-TGCTTGACTGAGTCGTCAGAAT-3′
mdp2 3′ mdp2-k 5′-AGAAGAGGAGGACCGAGTGG-3′
mdp2 3′ mdp2-l 5′-ATCAGTCTCTGAGGGAAATAGGC-3′