Figure 2From: A permissive chromatin structure is adopted prior to site-specific DNA demethylation of developmentally expressed genes involved in macronuclear differentiationLocalization of different histone PTMs in parental macronuclei (p) and micronuclei (m) of exconjugant cells shortly after separation. (A-C) In situ staining using antibodies directed against H3K9me3/K27me3 (A, hot magenta), H3K4me (B, hot blue), H3K9ac/K14ac (C, yellow). DNA counterstaining with To-Pro-3 (red). (D, E) Distribution of these PTMs on the 5′- and 3′-subtelomeric regions of mdp1 as well as mdp2 nanochromosomes during vegetative growth and in exconjugant cells.Back to article page