Cooperative function of H2A.Z, WDR5 and BPTF. (A) After silencing H2A.Z, WDR5 or BPTF in LD611 cells, the mRNA levels of the 10 genes, which show higher H2A.Z levels around the transcription start site (TSS) and augmented expression, were quantified by qRT-PCR. The values are expressed as fold changes from the levels in undepleted cells. (B) MTT proliferation assays were carried out in triplicate using LD611 cells depleted of H2A.Z, WDR5 or BPTF as indicated. (C) LD611 cells depleted of H2A.Z, WDR5 or BPTF were seeded onto the upper chamber, and then allowed to migrate toward 10% fetal bovine serum (FBS) in the lower chamber. The migrated cells were photographed and counted. The Y axis indicates the relative percentage of the migrated cell. Three independent experiments in triplicate wells were performed. MTT assay, 3–4, 5-(dimethyl-thyazol-2-yl)-2, 5-diphenyltetrazolium assay.