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Table 1 Bisulfite amplicon sequencing (BSAS) accuracy improvement

From: Focused, high accuracy 5-methylcytosine quantitation with base resolution by benchtop next-generation sequencing

Control (% 5-mC)

Rat

Mouse

0

5.41

30.51

5

2.61

1.53

10

1.79

10.01

25

3.05

1.03

50

12.17

25.47

75

7.33

40.79

100

3.81

4.78

Overall

5.16864

16.3067

  1. Mean squared error (MSE) improvements of methylation quantitation from BSAS compared to Sanger/epigenetic sequencing methylation analysis (ESME) from each methylation control are presented. BSAS was more accurate at quantifying methylation at every control, and demonstrated an overall 16-fold decrease in error compared to Sanger/ESME across the mouse controls and 5-fold decrease in error across the rat controls.