Control (% 5-mC) | Rat | Mouse |
---|
0 | 5.41 | 30.51 |
5 | 2.61 | 1.53 |
10 | 1.79 | 10.01 |
25 | 3.05 | 1.03 |
50 | 12.17 | 25.47 |
75 | 7.33 | 40.79 |
100 | 3.81 | 4.78 |
Overall | 5.16864 | 16.3067 |
- Mean squared error (MSE) improvements of methylation quantitation from BSAS compared to Sanger/epigenetic sequencing methylation analysis (ESME) from each methylation control are presented. BSAS was more accurate at quantifying methylation at every control, and demonstrated an overall 16-fold decrease in error compared to Sanger/ESME across the mouse controls and 5-fold decrease in error across the rat controls.