|
Control (% 5-mC)
|
Rat
|
Mouse
|
|---|
|
0
|
5.41
|
30.51
|
|
5
|
2.61
|
1.53
|
|
10
|
1.79
|
10.01
|
|
25
|
3.05
|
1.03
|
|
50
|
12.17
|
25.47
|
|
75
|
7.33
|
40.79
|
|
100
|
3.81
|
4.78
|
|
Overall
|
5.16864
|
16.3067
|
- Mean squared error (MSE) improvements of methylation quantitation from BSAS compared to Sanger/epigenetic sequencing methylation analysis (ESME) from each methylation control are presented. BSAS was more accurate at quantifying methylation at every control, and demonstrated an overall 16-fold decrease in error compared to Sanger/ESME across the mouse controls and 5-fold decrease in error across the rat controls.
