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Table 1 Bisulfite amplicon sequencing (BSAS) accuracy improvement

From: Focused, high accuracy 5-methylcytosine quantitation with base resolution by benchtop next-generation sequencing

Control (% 5-mC) Rat Mouse
0 5.41 30.51
5 2.61 1.53
10 1.79 10.01
25 3.05 1.03
50 12.17 25.47
75 7.33 40.79
100 3.81 4.78
Overall 5.16864 16.3067
  1. Mean squared error (MSE) improvements of methylation quantitation from BSAS compared to Sanger/epigenetic sequencing methylation analysis (ESME) from each methylation control are presented. BSAS was more accurate at quantifying methylation at every control, and demonstrated an overall 16-fold decrease in error compared to Sanger/ESME across the mouse controls and 5-fold decrease in error across the rat controls.