Figure 5

Confidence interval sequencing saturation. The theoretical confidence interval based on the binomial model of methylation quantitation for measurement of 50% cytosine methylation for both the (A) mouse and (B) rat methylation controls (solid black curves), where the empirical data are represented as horizontal lines (black) and the average of the empirical data (red line). A conservative confidence interval (dashed curves) is also shown, representing the theoretical model with 0.1n with n being the actual depth of the experimental data.