Rho promoter methylation in mouse retina and cerebellum. (A) Quantitative PCR showed Rho mRNA expression to be high in the mouse retina (n = 4) while expression was not detected in the mouse cerebellum (n =4). (B) Rho promoter methylation measured by bisulfite amplicon sequencing (BSAS) in both the cerebellum (white) and the retina (black) across 13 CpG sites in the Rho promoter. (C) Rho promoter methylation measured by Sanger sequencing in the cerebellum and retina across 13 CpG sites in the Rho promoter. Error bars = SD. ***P <0.001, **P <0.002; t test.