Table 1 Histone H3 mutants exhibiting altered rapamycin sensitivity
Histone H3 residue | Rapamycin phenotype | Nucleosome location | Source |
|---|---|---|---|
A1S | −1 | Tail | This study |
T3A | +1 | Tail | This study |
T3D | 0 | Tail | This study |
Q5A | +2 | Tail | This study |
Q5E | +2 | Tail | This study |
K14A | −2 | Tail | [36] |
K14R | 0 | Tail | This study |
K14Q | 0 | Tail | This study |
R17A | −2 | Tail | This study |
R17K | −2 | Tail | This study |
K18A | −1 | Tail | [36] |
K18R | −2 | Tail | This study |
K18Q | 0 | Tail | This study |
K23A | −1 | Tail | [36] |
K23R | 0 | Tail | This study |
K23Q | 0 | Tail | This study |
S28A | −2 | Tail | This study |
S28D | 0 | Tail | This study |
P30A | −1 | Tail | This study |
K37A | Lethal | Tail | This study |
K37R | 0 | Tail | This study |
K37Q | 0 | Tail | This study |
R49A | −2 | Lateral | This study |
R49K | 0 | Lateral | This study |
K56A | −3 | Lateral | [36] |
K56R | −2 | Lateral | [36] |
K56Q | +2 | Lateral | [36] |
S57A | +1 | Disk | [36] |
S57D | +1 | Disk | [36] |
E73A | −1 | Disk | This study |
E73Q | −1 | Disk | This study |
Q76A | −1 | Disk | This study |
Q76E | −1 | Disk | This study |
T80A | +1 | Disk | This study |
T80D | −1 | Disk | This study |
L82A | −1 | Disk | This study |
R83A | −2 | Lateral | This study |
R83K | 0 | Lateral | This study |
Q85A | −2 | Lateral | This study |
Q85E | 0 | Lateral | This study |
S86A | −2 | Lateral | This study |
S86D | −2 | Lateral | This study |
L92A | +1 | Buried | This study |
E105A | −1 | Disk | This study |
A110S | −1 | Buried | This study |
A111S | −1 | Buried | This study |
A114S | −1 | Disk | This study |
V117A | −3 | Lateral | This study |
Q120A | +1 | Lateral | This study |
K121A | −1 | Disk | This study |
K121R | 0 | Disk | This study |
K121Q | −1 | Disk | This study |
K122A | −1 | Disk | This study |
K122R | −3 | Disk | This study |
K122Q | −1 | Disk | This study |
R129A | +1 | Disk | This study |
R129K | 0 | Disk | This study |
Total number of H3 mutants screened | 114 | ||
Number of H3 mutants with phenotype | 30 | ||
Percentage of total H3 mutants with phenotype | 26.3% | ||
Percentage of H3 mutants with resistant phenotypes | 26.7% | ||
Percentage of H3 mutants with sensitive phenotypes | 73.3% | ||