The effect of TORC1 inhibition on Hmo1 and Nhp10 localization in H3WT and H3K37A. (A) H3WT and H3K37A strains expressing Hmo1-6XHA were mock treated or treated with 25 nM rapamycin for 30 minutes before staining with rhodamine-conjugated α-HA antibody and DAPI. (B) As (A) except Nhp10-6XHA expressing cells were treated with 25 nM rapamycin for two hours. Cells were analyzed by confocal microscopy as described in the Methods and the data are representative of four independent experiments. Scale bar indicates 5 μm. Rap, rapamycin.