Selective effects of histone H3 residues on TORC1-regulated cell growth. (A) Subset of histone H3 residues identified in chemical genomics screen. Equal numbers of cells were 5-fold serially diluted and spotted to control (yeast extract/peptone/dextrose (YPD)) or YPD containing 25 nM rapamycin plates. Plates were incubated at 30°C for 4 days before photographing. (B) As (A), except cells were spotted to control YPD or YPD plates containing 12 mM caffeine. (C) A hyperactive Tor1 kinase (Tor1A1957V) rescues H3K14A and H3K37A growth on rapamycin. The indicated strains were transformed with control vector or vector expressing Tor1A1957V and spotted to SC-leucine or SC-leucine containing 25 nM rapamycin. Plates were incubated at 30°C for 4 days. (D) Sch9 signaling rescues the H3K14A, but not H3K37A, TORC1 phenotype. The indicated strains were transformed with control vector or vector expressing Sch92D3E, spotted to either SC-uracil or SC-uracil containing 25 nM rapamycin, and incubated as in (D). (E) H3S57A mutants are sensitive to decreased protein kinase A (PKA) signaling. Cells carrying control vector or a high copy PDE2 expression vector were spotted to SC-leucine plates and incubated at 30°C for 4 days. leu, leucine; Rap, rapamycin; SC, synthetic complete medium; ura, uracil; WT, wild-type.