Evidence for broad feedback regulation by AP-1 and NF-κB family members, and c-Myc. (A) Statistical enrichments of AP-1 and NF-κB binding sites link these transcription factors to activated enhancers and the upregulated epithelial-mesenchymal transition-related gene clusters (EMT-GCs). EMT clusters themselves are enriched for in pathways and functions associated with positive regulation of AP-1 and NF-κB. Some genes in GC16 and GC19 that are known to regulate either AP-1 or NF-κB are listed. (B) c-Myc binding sites are enriched in repressed enhancers and the repressed EMT gene cluster, GC15. Moreover, GC16 is enriched for genes that are repressed by c-Myc.