Figure 3

MERVL ERVs are derepressed in G9a- and GLP-deficient mESCs and MERVL silencing is dependent on G9a catalytic activity. (A) Upregulation of MERVL in G9a and GLP KO mESCs. Expression of MERVL, MMERVK10C and IAPEz ERVs in TT2 wt, G9a and GLP KO mESCs was analyzed by qRT-PCR. Mean (+/-SD) expression levels relative to the wt line for three technical replicates (normalized to β-actin) are shown. (B) Catalytic activity of G9a but not GLP is required for MERVL silencing. G9a or GLP KO mESCs stably expressing wt or catalytic mutant G9a (C1168A) or GLP (C1201A) [50] transgenes, respectively, were assessed for MERVL expression by qRT-PCR, as described above. (C) MERVL but not MMERVK10C or IAPEz ERVs are upregulated upon KD of Glp. Relative expression of ERVs was determined by qRT-PCR, as described above. (D) Efficiency of each KD was determined by qRT-PCR with primers specific for Kap1 and Glp, as described above. SD, standard deviation.