DNA methylation analysis by COBRA for the PEG10 and H19 DMRs, KERV-1 LTR and LINE1 5′ region in male pouch young germ and somatic cells at 7, 9, 12 and 14 days postpartum and in adult sperm. The gel pictures show the cut and uncut bands after digestion by the restriction enzymes indicated in each panel. The fluorescence positive and negative samples represent positively sorted presumed germ cells and negatively sorted somatic control cells. Adult sperm samples were labeled as ‘Sp’. The vertical axes of bar graphs represent ratio of the intensity of the cut bands reflecting the methylation level of each sample. In C and D, the regions and bands subjected to cut/uncut intensity calculation were labeled by U for uncut and C for cut, respectively. (A) PEG10 DMR, (B) H19 DMR, (C) KERV-1 LTR, (D) LINE1 5′ region.