Figure 2
From: Postnatal epigenetic reprogramming in the germline of a marsupial, the tammar wallaby
DNA methylation status of the H19 DMR, KERV-1 LTR and LINE1 5′ region in male pouch young germ cells at 1, 5, 10 and 14 days postpartum. (A) Single cell suspension of the D5 tammar pouch young gonads labeled by the SSEA1 antibody. The white arrows indicate some of presumed germ cells labeled very clearly. (B) The black and white circles represent methylated and unmethylated CpG sites, respectively. The KERV-1 and LINE1 data only show the number of methylated CpG in each sequence as CpG sites are not always conserved among clones because of the heterogeneous amplification of these repetitive sequences. No data for ‘n.d.’. (C) The vertical axis for the graph of H19 DMR methylation represents the percentage of methylated CpG sites based on the data shown in Figure 2B. In the graphs of retrotransposon methylation, the vertical axes represent total number of methylated CpG sites in each sequence instead of percentage. Blue horizontal bars indicate average of the data.