Figure 7

Conservation of bipartite recognition of H3K36me nucleosomes. (a) The PWWP domains of HDGF2 (3QBY), MSH6 (2GFU), DNMT3B (1KHC), BRPF1 (2X4X) and WHSC1L1 (2DAQ) were aligned with the PSIP1-PWWP domain in our model of the PWWP-H3K36me3 nucleosome complex. Detailed view showing H3 residues 31 to 38 as balls-and-sticks, the DNA in cartoon representation and the van der Waals surface of the PWWP domain color coded by electrostatic potential. (b) The chromodomain of MRG15 (2F5K) was manually docked on the H3K36me3 nucleosome model, positioning the aromatic cage around H3K36me3 and orienting its basic patches towards the DNA, while minimizing interatomic clashes.