H3K36 methylation-dependent nucleosome binding by the PSIP1-PWWP domain. (a) Immunoblot analysis of GST pull-downs with the indicated GST-fusion proteins incubated with mono-nucleosomes extracted from (mutant) yeast strains probed for histone H3. (b) GST pull-downs with nucleosomes isolated from indicated (mutant) yeast strains. (c) Immunoblot analysis of pull-downs with GST-PWWP lysates and the indicated biotinylated histone H3 peptides probed for GST. (d) GST pull-down assay for GST-PWWP (PWWP), GST-PWWP including flanking AT-hook (PWWP+AT) or control proteins (TAF3, UbcH5B) were incubated with HeLa nucleosomes. Eluted proteins were detected by immunoblots with the indicated antibodies.