Skip to main content
Figure 8 | Epigenetics & Chromatin

Figure 8

From: The histone deacetylase inhibitor sodium valproate causes limited transcriptional change in mouse embryonic stem cells but selectively overrides Polycomb-mediated Hoxb silencing

Figure 8

Treatment of differentiating embryonic stem cells with retinoic acid increases H3K9ac at Hoxb genes. Chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) was used to investigate H3K9ac levels at two or three regions across four Hoxb genes, as indicated, in differentiating embryonic stem (ES) cells (CCE/R) before and after treatment with retinoic acid (RA). Leukaemia inhibitory factor (LIF) was removed at d0 (0 h) and RA added at d2. Location of the primer pairs used is shown in Figure 5B. Data are displayed as the enrichment of the Hoxb sequences in the bound relative to the unbound (B/UB) sample for each timepoint. Error bars represent the standard error of the mean of independent experiments (n = 2).

Back to article page

Epigenetics & Chromatin

ISSN: 1756-8935