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Figure 1 | Epigenetics & Chromatin

Figure 1

From: The histone deacetylase inhibitor sodium valproate causes limited transcriptional change in mouse embryonic stem cells but selectively overrides Polycomb-mediated Hoxb silencing

Figure 1

Valproic acid induces global histone hyperacetylation and H3K4 hypermethylation in embryonic stem cells. (A) Western blots showing changes in the indicated histone modifications after treatment of embryonic stem (ES) cells (CCE/R) for up to 8 h with 1 mM valproic acid (VPA), and at various timepoints after its removal (wash out). Staining with an antibody to the unmodified H3 C-terminal domain was used as a loading control (H3 C-term, bottom tracks). (B) Measurement of the fluorescent secondary antibody signal quantifies changes in histone modifications over time (n = 3).

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Epigenetics & Chromatin

ISSN: 1756-8935