Figure 3
From: Crosstalk between SET7/9-dependent methylation and ARTD1-mediated ADP-ribosylation of histone H1.4
Linker histones are methylated by SET7/9. (A) HIS-tagged H1.4 FL and a deletion mutant without the CTD (ΔCTD) were in vitro methylated by SET7/9 in the presence or absence of plasmid DNA. (B) Schematic depiction of H1.4 wild-type (WT) protein and constructs. NTD, N-terminal domain; GD, globular domain; CTD, C-terminal domain; ΔCT, H1.4 construct completely lacking the C-terminus. (C) Recombinant HIS-tagged H1.4 full-length (FL) and deletion mutants were methylated in vitro by SET7/9. (D) H1.4 C-terminal fragments were expressed as GST fusion proteins and methylated in vitro by SET7/9. Methylation was analyzed by autoradiography.