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Table 2 Frequency of methylation by methylation-specific polymerase chain reaction in the 96 samples (48 tumor and matching lung tissue) in five fragments

From: Genome-wide DNA methylation profiling of non-small cell lung carcinomas

 

CDKN2A

Frag_01

Frag_02

RASSF1

ZIC4

count (n)

Sensitivity (%)

Specificity (%)

Count (n)

Sensitivity (%)

Specificity (%)

Count (n)

Sensitivity (%)

Specificity (%)

Count (n)

Sensitivity (%)

Specificity (%)

Count (n)

Sensitivity (%)

Specificity (%)

Lung

24/48

75

27

0/47

62

100

9/48

75

56

0/41

42

100

0/39

77

100

Tumor

36/48*

29/47**

36/48**

17/41**

30/39**

Adenocarcinoma

13/17

76.5

46.4

5/16

31.3

27.7

12/17

70.6

46.1

8/15

53.3

53.3

7/13

53.9

36.8

Squamous cell carcinoma

15/16

93.8

53.6

13/16

81.3**

72.2

14/16

87.5

53.9

7/16

43.8

46.7

12/16

75.0

63.2

  1. Sensitivity is calculated using the ratio between methylated (amplified) sample and the total amount of samples analyzed per fragment; specificity is the ratio of the difference between amplification in tumor and in the matching lung sample (or adenocarcinoma versus squamous cell carcinoma). Statistical significance of the difference between control lung samples and tumors, and between adenocarcinomas and squamous cell carcionmas were calculated using chi-square. *P<0.02; **P 0.0001.