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Table 2 Frequency of methylation by methylation-specific polymerase chain reaction in the 96 samples (48 tumor and matching lung tissue) in five fragments

From: Genome-wide DNA methylation profiling of non-small cell lung carcinomas

  CDKN2A Frag_01 Frag_02 RASSF1 ZIC4
count (n) Sensitivity (%) Specificity (%) Count (n) Sensitivity (%) Specificity (%) Count (n) Sensitivity (%) Specificity (%) Count (n) Sensitivity (%) Specificity (%) Count (n) Sensitivity (%) Specificity (%)
Lung 24/48 75 27 0/47 62 100 9/48 75 56 0/41 42 100 0/39 77 100
Tumor 36/48* 29/47** 36/48** 17/41** 30/39**
Adenocarcinoma 13/17 76.5 46.4 5/16 31.3 27.7 12/17 70.6 46.1 8/15 53.3 53.3 7/13 53.9 36.8
Squamous cell carcinoma 15/16 93.8 53.6 13/16 81.3** 72.2 14/16 87.5 53.9 7/16 43.8 46.7 12/16 75.0 63.2
  1. Sensitivity is calculated using the ratio between methylated (amplified) sample and the total amount of samples analyzed per fragment; specificity is the ratio of the difference between amplification in tumor and in the matching lung sample (or adenocarcinoma versus squamous cell carcinoma). Statistical significance of the difference between control lung samples and tumors, and between adenocarcinomas and squamous cell carcionmas were calculated using chi-square. *P<0.02; **P 0.0001.