Differentially methylated region in non-small cell carcinoma versus paired lung tissue samples. (A) Heatmap of 14,742 most significant DMRs, picturing regions with mostly hypermethylated (top), mostly hypomethylated (middle), and mixes (bottom). Color bar at the bottom represents the log ratio of the normalized signal in tumor versus. lung (red= hypermethylation, green= hypomethylation). Dashed line represents cut in the dendogram, generating nine groups of DMRs. The DMRs from the two most distinctive groups are depicted on the chromosomes. Red: cluster containing regions hypermethylated in all samples; green: cluster containing regions hypomethylated in all samples. (B) Bar plot showing mean enrichment of DMRs at chromosome ends calculated as ratio between proportion of 1 Mbp region at chromosome ends and total proportion of each chromosome covered by DMRs. Error bars show standard error of mean of all chromosomes. Statistical testing was done against random regions using one-tailed Student’s t-test (***P≤0.0001). (C) DMRs distribution relative to gene position. Number of hyper- and hypomethylated genes and regions (outside gene area), and their distribution as in promoter, gene body and 3′ UTR (top panel: all seven samples; middle panel: squamous cell carcinomas, bottom panel: adenocarcinoma). (D): Gene function distribution of the differentially methylated genes showing number of hyper- and hypomethylated genes relative to gene classes or function. DMR: differentially methylated regions; UTR: untranslated region.