Skip to main content

Table 4 Oligos

From: The male germ cell gene regulator CTCFL is functionally different from CTCF and binds CTCF-like consensus sites in a nucleosome composition-dependent manner

Oligos used for RACE PCR

Name

Forward

Backward

RACE

GGACACTCGTATTTGGGCACATTC

CACAGGGAGCACTTGAAGGGCTTC

Oligos used in real-time PCR for ChIP

Name

Forward

Backward

Gal3st1

TCCTGGGTGAGGTCAGGAAG

GGAACTCCGAGTAGCTTCAATG

Stra8

TCCTAGAGAAGGGGGTGTTACC

AGCTGACCACCACACGTTTTC

Prss50

AGAGGAGGGTAGGGGTATCGAC

TCGCCTCAGCTAATTTCTAAGC

Vps18

CTGCTGCAGTTCCTCATGTTG

GTGTGACAGATGGAGGAGCAC

Chr10

AAGGTTGGTAGCTCTGCTTGGACTGCTCG

AATGTCACAAGCAAAGAAAAGCACGCAAAT

Amylase

AATTCTCCTTGTACGGCTTCGTG

TAGCAATGATGTGCACAGCTGAA

Oligos used in real-time PCR for RNA expression

Name

Forward

Backward

Clgn

TGTCTTCCTTACTCTTCTCTTCCG

GAAGCCAGGTGAAGCTGAGGTC

Ctcf

CCACCTGCCAAGAAGAGAAGG

GCACCTGTATTCTGATCTTCGAC

Tcfl5

ACGAGATAGGAGGCGCAGAATC

GTTGTTGCTTTATCTGTCTCCG

Gal3st1testis-specific form

GCTACTCGGAGTTCCGGAAA

GACTTGCAGGGCTTCTTTGG

Gal3st1

ACTGTATCCCAACATGGCCTTC

ATATCTCGCCGAGGTTGACAC

Stra8

GGCAGTTTACTCCCAGTCTGATA

CAACTTATCCAGGCTTTCTTCCT

Prss50

GACAGTTCTCTCTGCACTGTGAC

CACATTTCTTGCTGTTCAGGATA

Ctcfl

GCTCTGGCTGTGCACCTTACG

CCCACTGTGCCACCATCATC

Ccna1

GAGTATGCAGAGGAGATTCATCG

TCATGTAGTGAGCCTTGGGTCTG

lpcat2

AGCACCCAGTGAGGAAGAGA

TTCGTAGGTGTGATCCGTCA

itfg3

ACGAGGTGTCTTCTGCCTGT

GTTCCCACTAAAGCTGCTGG

dio2

TGCAGATCCTGCCAGTCTTT

CACACTGGAATTGGGAGCAT

hgf

GATGAGTGTGCCAACAGGTG

GGTCAAATTCATGGCCAAAC

akr1c18

CCAGGCCATTCTAAGCAAGA

TCAGGGAATTTTCCAAGCTG

Oligos used in EMSA

Name

Forward

Backward

Stra8

GGATCTGTGCTGTGTGTCCTCCTCGACTCCT

CCTCTAGGAGTCGCAAGTGACCCACACATGCATGC

 

AGAGCATGCATGTGTGGGTCACTTGCGACTC

 
 

CTAGAGGA

TCTAGGAGTCGAGGAGGACACACAGCACAGATCCT

Prss50

ATCTAGGGGGCGCCACGCAGGCTGGGCACC

CCACAATGGCGCCCTCCATCGGGCGCCTCATGGT

 

AGCGCACCATGAGGCGCCCGATGGAGGGCG

GCGCTGGTGCCCAGCCTGCGTGGCGCCCCCTAGATG

 

CCATTGTGGA

 

Oligos used for generating transgenic mice and cells

Name

 

Oligo 1

5-TCTTTTTCCATCAGGGGTCGTCAC-3

Oligo 2

5-GAGAAGCGCGATCACATGGTCCTG-3

Oligo 3

5-GCACCGTTTGCAGGGTCAGGATC-3

Oligo 4

5-TCCAAATCACAGCGCCACCTACAG-3

Oligo 5

5-GGTTCTTAGAGATAGGGTTTCTCTG-3

Oligo 6

5-GGTGTTCTGCTGGTAGTGGTC-3

Oligo 7

5-CGGCATCAGAGCAGCCGATTG-3

Oligo 8

5-GTTATGATCTGGGTATCGTCCACTG-3