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Table 3 Rescue of CTCF-deficient ES cells by exogenously introduced GFP-CTCF(L)

From: The male germ cell gene regulator CTCFL is functionally different from CTCF and binds CTCF-like consensus sites in a nucleosome composition-dependent manner

Species

Construct

Deletion in ES cells**

Partial deletion in ES cells**

No deletion in ES cells**

Functional CTCF substitution

N.a.*

GFP-CRE

0% (0/65)

28% (18/65)

72% (47/65)

No

Mouse

GFPCTCFL-

0% (0/18)

50% (9/18)

50% (9/18)

No

Mouse

CTCFGFP- CRE

83% (19/23)

0% (0/23)

17% (4/23)

Yes

Mouse

GFPCTCF- CRE

95% (40/42)

2% (1/42)

2% (1/42)

Yes

Chicken

CTCFYFP- CRE

90% (37/41)

2% (1/41)

7% (3/41)

Yes

  1. *: Not applicable.
  2. **: Percentage and number of clones (between brackets) in which the conditional CTCF alleles were deleted, partially deleted or not deleted are shown.