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Table 2 Yeast strains used in this study

From: The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability

Strain

Genotypea,b

Parent, source or referencec

RMY102

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 [pRM102]

Mann, 1992

SNY089

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 asf1::KANMX6 [pRM102]

RMY102

SNY093

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 asf1::KANMX6 [pEMHE81]

SNY089

SNY090

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 [pRM102]

RMY102

SNY091

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 [pEMHE81]

SNY090

SNY092

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 [pEMHE81H4G94A]

SNY090

SNY095

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 [pEMHE81H4G94P]

SNY090

MCY073

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 thr- hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 RTT109:: 6xGly-(FLAG)3::hphMX6 [pEMHE81]

SNY091

MCY074

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 RTT109::6xGly-(FLAG)3::hphMX6 [pEMHE81H4G94A]

SNY092

MCY075

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 RTT109::6xGly-(FLAG)3::hphMX6 [pEMHE81H4G94P]

SNY095

MCY076

MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr- hht1 hhf1::LEU2 hht2 hhf2::HIS3 asf1::KANMX6 RTT109::6xGly-(FLAG)3::hphMX6 [pEMHE81]

SNY093

W1588-4a

MATα leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1

Gift from R Rothstein

W1588-4c

MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1

Gift from R Rothstein

BKD215

MATα leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht1 hhf1:: KANMX6

W1588-4a (see Methods)

BKD203

MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht2 hhf2::HHT2 HHF2::TRP1

W1588-4c (see Methods)

BKD204

MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht2 hhf2::HHT2 HHF2::TRP1

W1588-4c (see Methods)

BKD207

MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht2 hhf2::HHT2 HHF2 G94P::TRP1

W1588-4c (see Methods)

BKD210

MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht2 hhf2::HHT2 HHF2 G94A::TRP1

W1588-4c (see Methods)

MCY081

MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 HHF2::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6

BKD203 × BKD215

MCY084

MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 HHF2::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6 [pRM102]

MCY081

MCY091

MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 HHT2 HHF2::TRP1 hht1 hhf1:: KANMX6 [pRM102]

Segregant from MCY081

MCY082

MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 hhf2G94A::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6

BKD210 × BKD215

MCY086

MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 hhf2G94A::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6 [pRM102]

MCY082

MCY094

MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 HHT2 hhf2G94A::TRP1 hht1 hhf1:: KANMX6 [pRM102]

Segregant from MCY082

MCY083

MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 hhf2G94P::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6

BKD207 × BKD215

MCY088

MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 hhf2G94P::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6 [pRM102]

MCY083

MCY097

MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 HHT2 hhf2G94P::TRP1 hht1 hhf1:: KANMX6 [pRM102]

Segregant from MCY088

MCY021

MATα leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht1 hhf1:: KANMX6 asf1:: his5 + MX6

BKD215

MCY043

MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 asf1:: his5 + MX6

Segregant from BKD207 × MCY021

  1. aPlasmids are indicated in square brackets.
  2. bStrains derived from the same parent are grouped together.
  3. cUnless noted, strains are from this study.
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Epigenetics & Chromatin

ISSN: 1756-8935