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Table 2 Yeast strains used in this study

From: The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability

Strain Genotypea,b Parent, source or referencec
RMY102 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 [pRM102] Mann, 1992
SNY089 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 asf1::KANMX6 [pRM102] RMY102
SNY093 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 asf1::KANMX6 [pEMHE81] SNY089
SNY090 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 [pRM102] RMY102
SNY091 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 [pEMHE81] SNY090
SNY092 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 [pEMHE81H4G94A] SNY090
SNY095 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 [pEMHE81H4G94P] SNY090
MCY073 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 thr- hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 RTT109:: 6xGly-(FLAG)3::hphMX6 [pEMHE81] SNY091
MCY074 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 RTT109::6xGly-(FLAG)3::hphMX6 [pEMHE81H4G94A] SNY092
MCY075 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr-hht1 hhf1::LEU2 hht2 hhf2::HIS3 ASF1::13MycKANMX6 RTT109::6xGly-(FLAG)3::hphMX6 [pEMHE81H4G94P] SNY095
MCY076 MATa ade2-101 his3∆200 lys2-801 trp1∆901 ura3-52 Thr- hht1 hhf1::LEU2 hht2 hhf2::HIS3 asf1::KANMX6 RTT109::6xGly-(FLAG)3::hphMX6 [pEMHE81] SNY093
W1588-4a MATα leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 Gift from R Rothstein
W1588-4c MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 Gift from R Rothstein
BKD215 MATα leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht1 hhf1:: KANMX6 W1588-4a (see Methods)
BKD203 MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht2 hhf2::HHT2 HHF2::TRP1 W1588-4c (see Methods)
BKD204 MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht2 hhf2::HHT2 HHF2::TRP1 W1588-4c (see Methods)
BKD207 MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht2 hhf2::HHT2 HHF2 G94P::TRP1 W1588-4c (see Methods)
BKD210 MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht2 hhf2::HHT2 HHF2 G94A::TRP1 W1588-4c (see Methods)
MCY081 MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 HHF2::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6 BKD203 × BKD215
MCY084 MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 HHF2::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6 [pRM102] MCY081
MCY091 MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 HHT2 HHF2::TRP1 hht1 hhf1:: KANMX6 [pRM102] Segregant from MCY081
MCY082 MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 hhf2G94A::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6 BKD210 × BKD215
MCY086 MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 hhf2G94A::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6 [pRM102] MCY082
MCY094 MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 HHT2 hhf2G94A::TRP1 hht1 hhf1:: KANMX6 [pRM102] Segregant from MCY082
MCY083 MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 hhf2G94P::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6 BKD207 × BKD215
MCY088 MATa /MATα leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 ura3-1/ura3-1 trp1-1/trp1-1 HHT2 hhf2G94P::TRP1/HHT2 HHF2 HHT1 HHF1/hht1 hhf1:: KANMX6 [pRM102] MCY083
MCY097 MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 HHT2 hhf2G94P::TRP1 hht1 hhf1:: KANMX6 [pRM102] Segregant from MCY088
MCY021 MATα leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 hht1 hhf1:: KANMX6 asf1:: his5 + MX6 BKD215
MCY043 MATa leu2-3,112 ade2-1 can1-100 his3-11,15 ura3-1 trp1-1 asf1:: his5 + MX6 Segregant from BKD207 × MCY021
  1. aPlasmids are indicated in square brackets.
  2. bStrains derived from the same parent are grouped together.
  3. cUnless noted, strains are from this study.