Table 1 Plasmids used in this study

pFA6a-KANMX6

Longtine, 1998

pFA6a-his5 + MX6

Longtine, 1998

pRS314

CEN6 ARSH4 TRP1

Sikorski & Hieter, 1989

pRS414

CEN6 ARSH4 TRP1

Sikorski & Hieter, 1989

pRS316

CEN6 ARSH4 URA3

Sikorski & Hieter, 1989

pRM102

CEN4 ARS1 p(GAL10)-HHT2 p(GAL1)-HHF2 URA3

Mann & Grunstein, 1992

pEMHE81

pRS414 containing HHT2 and HHF2

Hyland, 2005

pEMHE81H4G94P

pEMHE81 containing HHT2 and hhf2 G94P

This study

pEMHE81H4G94A

pEMHE81 containing HHT2 and hhf2 G94A

This study

pRS314-Asf1-Myc

pRS314 containing 13 × C-terminally Myc tagged Asf1

English, 2006

pST39T60xtal

Triple expression vector for Asf1-H3/H4

English, 2006

pST39T60 H4^G94P

pST39T60xtal containing H4G94P mutation

This study

pET3a H4^T71C

Containing histone H4 T71C mutation

Park, 2004

pET3a H4^T71C,G94P

pET3a H4^T71C containing H4G94P mutation

This study

pET3a H4 ^{T71C, ∆94}

pET3a H4^T71C containing H4truncated after 94

This study

pET-60-DEST

GST-His₆-tag expression vector

Invitrogen

pET-60-yAsf1FL

Saccharomyces cerevisiae ASF1 inserted between the GST tag and the His₆ tag coding sequences. The Asf1 -1 position (pro) was changed to (cys) for fluorophore attachment, and production of yAsf1*⁵³²

Donham, 2011