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Table 1 Plasmids used in this study

From: The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability

Plasmid Characteristics Source or reference
pFA6a-KANMX6   Longtine, 1998
pFA6a-his5 + MX6   Longtine, 1998
pRS314 CEN6 ARSH4 TRP1 Sikorski & Hieter, 1989
pRS414 CEN6 ARSH4 TRP1 Sikorski & Hieter, 1989
pRS316 CEN6 ARSH4 URA3 Sikorski & Hieter, 1989
pRM102 CEN4 ARS1 p(GAL10)-HHT2 p(GAL1)-HHF2 URA3 Mann & Grunstein, 1992
pEMHE81 pRS414 containing HHT2 and HHF2 Hyland, 2005
pEMHE81H4G94P pEMHE81 containing HHT2 and hhf2 G94P This study
pEMHE81H4G94A pEMHE81 containing HHT2 and hhf2 G94A This study
pRS314-Asf1-Myc pRS314 containing 13 × C-terminally Myc tagged Asf1 English, 2006
pST39T60xtal Triple expression vector for Asf1-H3/H4 English, 2006
pST39T60 H4G94P pST39T60xtal containing H4G94P mutation This study
pET3a H4T71C Containing histone H4 T71C mutation Park, 2004
pET3a H4T71C,G94P pET3a H4T71C containing H4G94P mutation This study
pET3a H4 T71C, ∆94 pET3a H4T71C containing H4truncated after 94 This study
pET-60-DEST GST-His6-tag expression vector Invitrogen
pET-60-yAsf1FL Saccharomyces cerevisiae ASF1 inserted between the GST tag and the His6 tag coding sequences. The Asf1 -1 position (pro) was changed to (cys) for fluorophore attachment, and production of yAsf1*532 Donham, 2011