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Figure 6 | Epigenetics & Chromatin

Figure 6

From: The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability

Figure 6

Lack of the H4 C-terminal tail but not H4G94Palters anti-silencing function 1 (Asf1)-mediated disome formation. H3/H4G94P(A) or H3/H4∆94(B) were compared to wild-type (WT) H3/H4, all at 0.8 μM dimer concentration, for their ability to form tetrasomes and disomes on 80 bp 5 S DNA (0.4 μM) in the absence and presence of Asf1 (0, 0.8, 2.0 μM). Upper panels show images of SYBR Green I stained DNA, and lower panels show the quantitation of the amount of disomes and tetrasomes formed for each type of histone, from at least three independent experiments. Tetrasome and disome levels were normalized to the WT H3/H4 sample in the absence of Asf1.

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