Figure 2From: Lysine methyltransferase G9a is not required for DNMT3A/3B anchoring to methylated nucleosomes and maintenance of DNA methylation in somatic cellsG9a along with DNMT3A/3B binds to intact nucleosomal structures. Mononucleosomes released by extensive digestion with MNase were incubated in the absence (A) or presence (B) of 300 μg/ml EtBr for 10 min at room temperature, before loading onto the sucrose density gradients (5% to 25%) containing 300 mM NaCl. In (A), absorbance of each fraction, read at 260 nm, has been plotted on the top to represent the amount of DNA sedimenting in each fraction. Gradients were fractionated and analyzed as described previously.Back to article page