Figure 2

Control experiment demonstrating that the vibrations detected from undifferentiated human embryonic stem cell (hESC) chromatin are not an artifact of whole cell movement or instrumental vibration. (A) In a single hESC nucleus, (B)-(C) two line scan experiments were acquired across the nuclear envelope (a structure which should not vibrate and is representative of whole cell movement) and (D)-(E) two line experiments were acquired across a heterogeneous chromatin region (a structure shown to vibrate in Figure 1A -F). (F)-(I) The intensity carpet recovered from performing a line scan across each structure depicted in (B)-(E) respectively. (J)-(K) Auto- and cross correlation analysis of the Gaussian track derived for nuclear envelope movement centered at two adjacent middle columns reveal only the diffusive decay of random motion. (L)-(M) Auto- and cross correlation analysis of the Gaussian track derived for chromatin density movement centered at two adjacent middle columns, reveal the characteristic discrete peaks of positive correlation which represent different frequency oscillations.