Figure 1

Extracting the frequency of chromatin movement. (A) hESC nucleus expressing EGFP stained with Hoechst 33342. (B) hESC chromatin density region selected for characterization. (C) Intensity profile of the line-scan acquired across the hESC chromatin density region as a function of time. (D) Schematic of the Gaussian fit and the corresponding track. (E) Autocorrelation analysis of the Gaussian track derived for the hESC chromatin density region (columns 13 to 20). The midpoint was defined as column 16 (red curve) and 17 (blue curve). Cross correlation analysis of these two curves (green). (F) The track’s center of mass standard deviation in the x-y plane (90 nm). (G) HELA nucleus expressing EGFP stained with Hoechst 33342. (H) HELA chromatin density region selected for characterization. (I) Intensity carpet of the line-scan acquired across the HELA chromatin density region. (J) Schematic of the Gaussian fit and the corresponding track. (K) Auto- and cross correlation analysis of the Gaussian track derived for the HELA chromatin density region (columns 7 to 13). (L) The track’s center of mass standard deviation in the x-y plane (30 nm). (M) NIH3T3 nucleus expressing EGFP stained with Hoechst 33342. (N) NIH3T3 chromatin density region selected for characterization. (O) Intensity carpet of the line-scan acquired across the NIH3T3 chromatin density region. (P) Schematic of the Gaussian fit to and the corresponding track. (Q) Auto- and cross correlation analysis of the Gaussian track derived for the NIH3T3 chromatin density region (columns 23 to 28). (R) The track’s center of mass standard deviation in the x-y plane (30 nm).