Figure 4

IRF1 hnRNA transcripts are enriched in the MEN1-depleted cell line. (a, b) qRT-PCR to quantitate IRF1 mRNA and pre-mRNA expression in shRNA-MEN1 and shRNA-NS cell lines that were uninduced or treated with IFN-γ. IRF1 expression was normalized to β-actin and fold change relative to the uninduced shRNA-NS condition was calculated. Error bars are standard error (n = 4). **P ≤ 0.01, *P ≤ 0.05. Numbers are the base pair location of the primers used on the IRF1 gene; see Figure 1c. (c) qRT-PCR to quantitate IRF1 pre-mRNA expression in shRNA-MEN1 cells transiently transfected with a plasmid expressing MEN1 or an empty vector. IRF1 expression, normalized to β-actin and fold change calculated as above, is presented relative to shRNA-NS cells (black bar) transiently transfected with empty vector and induced. Student's t-test determined significance. *P ≤ 0.05. IFN: interferon; MEN1: multiple endocrine neoplasia type 1; NS: nonsilencing; qRT-PCR: quantitative real time PCR.