The H3K4 dimethylation profile at IRF1 is altered by MEN1 depletion. (a-j) Crosslinked chromatin immunoprecipitation of shRNAmir-MEN1 (shRNA-MEN1) and shRNAmir-nonsilencing (shRNA-NS) cell lines treated with IFN-γ for 30 minutes or uninduced. The indicated antibodies were used and qPCR quantified the precipitate yield using primers spanning the IRF1 locus (see Figure 1c), reported as percent of input. Immunoglobulin G served as the negative control. P ≤ 0.05 for 2f, i. IFN: interferon; qPCR: quantitative PCR; MEN1: multiple endocrine neoplasia type 1.