Mutations in the Med8-Med18-Med20 submodule compromise H3K9 methylation at the centromeric dg promoter. ChIP analyses show that the level of H3K9 dimethylation at the centromeric dg promoter is reduced in med18 Δ and med20Δ mutants relative to wild-type. A clr4Δ strain was processed in parallel for comparison. *P <0.003. The strains for this figure were: WT (FY498), med18 Δ (MT42), med20 Δ (MT26), and clr4Δ (PG3423).