Table 1 Primers and TaqMan™ probes used for analysis of β-globin primary transcripts and β-actin mRNA levels
Primers | |
|---|---|
Rho F | CAGAACACTGGGGTTTGGCT |
Rho R | GGCCAGCACAATGATGAGG |
BetaH F | CCAGAGGTTCTTTGCGTCC |
BetaH R | CTCAGTCCTATAACTCCTGCCTA |
BetaA F | TACACTTCTGTTCCCCATCTCTC |
BetaA R | CCAAAGGACGCAAAGAACC |
Epsilon F | TAGGCAAAGAGTGAGCATTCG |
Epsilon R | GCCAGGACGATGATCAGGAT |
Beta-actin F | TGGACTCTGGTGATGGTGT |
Beta-actin R | TCTCTCTCGGCTGTGGTGG |
TaqMan™ probes | |
Rho | FAM-CTCCACACC(T-BHQ1)TGCTTACCCCATTG-PO4 |
BetaH | FAM-ACAACA(T-BHQ1)CAAGAAGAGCTTTGCCCAG-PO4 |
BetaA | FAM-CTGATCGTC(T-BHQ`)ACCCCTGGACCCAGA-PO4 |
Epsilon | FAM-CTGTGCTTGG(T-BHQ1)GGGAAGAGGGGAT-PO4 |
Beta-actin | FAM-TCTATGAAGGC(T-BHQ1)ACGCCCTCCCCC-PO4 |