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Table 1 Depletion of SIRT2 mRNAs or treatment with sirtuin inhibitors does not induce senescence of proliferating cells and does not inhibit RAF-induced senescence as determined by 5-bromo-2'-deoxyuridine (BrdU) incorporation

From: Deacetylation of H4-K16Ac and heterochromatin assembly in senescence

Experiment Insult 4-HT (20 nM) BrdU-positive cells (%)
siRNA No Target + < 1
   - 75
  SIRT2 + < 1
   - 89
Sirtuin inhibitors DMSO + < 1
  (1/1000) - 89
  Salermide + < 1
  (50 μM) - 18
  Sirtinol + < 1
  (25 μM) - 29
  1. WI-38hTERT/GFP-RAF-ER cells were treated with the indicated siRNAs or sirtuin inhibitors in the presence or absence of 20 nM 4-HT for 3 days and then with fresh medium for 4 days. Cells treated with siRNAs were incubated with BrdU for 24 hours and cells treated with sirtuin inhibitors for 3 days to determine the percentage of cells able to enter S phase under these conditions.