Experiment | Insult | 4-HT (20 nM) | BrdU-positive cells (%) |
---|
siRNA | No Target | + | < 1 |
| | - | 75 |
| SIRT2 | + | < 1 |
| | - | 89 |
Sirtuin inhibitors | DMSO | + | < 1 |
| (1/1000) | - | 89 |
| Salermide | + | < 1 |
| (50 μM) | - | 18 |
| Sirtinol | + | < 1 |
| (25 μM) | - | 29 |
- WI-38hTERT/GFP-RAF-ER cells were treated with the indicated siRNAs or sirtuin inhibitors in the presence or absence of 20 nM 4-HT for 3 days and then with fresh medium for 4 days. Cells treated with siRNAs were incubated with BrdU for 24 hours and cells treated with sirtuin inhibitors for 3 days to determine the percentage of cells able to enter S phase under these conditions.