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Table 1 Depletion of SIRT2 mRNAs or treatment with sirtuin inhibitors does not induce senescence of proliferating cells and does not inhibit RAF-induced senescence as determined by 5-bromo-2'-deoxyuridine (BrdU) incorporation

From: Deacetylation of H4-K16Ac and heterochromatin assembly in senescence

Experiment

Insult

4-HT (20 nM)

BrdU-positive cells (%)

siRNA

No Target

+

< 1

  

-

75

 

SIRT2

+

< 1

  

-

89

Sirtuin inhibitors

DMSO

+

< 1

 

(1/1000)

-

89

 

Salermide

+

< 1

 

(50 μM)

-

18

 

Sirtinol

+

< 1

 

(25 μM)

-

29

  1. WI-38hTERT/GFP-RAF-ER cells were treated with the indicated siRNAs or sirtuin inhibitors in the presence or absence of 20 nM 4-HT for 3 days and then with fresh medium for 4 days. Cells treated with siRNAs were incubated with BrdU for 24 hours and cells treated with sirtuin inhibitors for 3 days to determine the percentage of cells able to enter S phase under these conditions.