Figure 10
From: Deacetylation of H4-K16Ac and heterochromatin assembly in senescence
Oncogene-induced senescence is not universally associated with H4-K16Ac deacetylation and striking senescence-associated heterochromatin foci (SAHFs). A) DNA 4',6'-diamidino-2-phenylindole (DAPI) staining of representative nuclei and corresponding DAPI coefficient of variation (CV) values. B) Boxplots of DAPI CV (n > 60, from one experiment). *DNA compaction statistically different from Prolif. (P < 10-5, Welch t-test). C) Relative abundance of H4 acetylation states measured at the protein level on deconvoluted mass spectra. Error bars show SD of at least two biological replicates. D) Immunoblot showing the level of H4-K16Ac in acid-extracted histones. Loading control: Coomassie blue staining of histones. WI-38 SenRAF: WI-38hTERT/GFP-RAF-ER + 4-HT 20 nM (5 days); IMR-90 SenRAS: IMR-90/ER-RASval12 + 4-HT 100 nM (6 days); BJ SenRAS: BJ/ER-RASval12 + 4-HT 100 nM (5 days).