Figure 2
From: Cohesin is required for expression of the estrogen receptor-alpha (ESR1) gene
SMC3 or MED12 depletion significantly impairs the estrogen-regulated transcriptome. mRNA expression profiling of estrogen-regulated genes after SMC3 (A) or MED12 (B) depletion and 10 nM 17β-Estradiol (E2) treatment for 6 h. The heatmaps show log2-fold-changes in experiments control siRNA + 17β-Estradiol vs. control siRNA alone (E2), SMC3 (A) or MED12 (B) siRNAs vs. control siRNA (siRNA SMC3 or siRNA MED12) and SMC3 (A) or MED12 (B) siRNAs + 17β-Estradiol vs. control siRNA (siRNA SMC3 + E2 or siRNA MED12 + E2) (columns) for genes that are significantly (q < 0.05; q = P-values adjusted to False discovery rate regulated by estrogen (Fold-change, FC < −log2 (1.5) or FC > log2 (1.5)) (rows). The color keys range from red marking downregulated, to blue marking upregulated genes; mean values; n = 3.