| |
Area of minimal enclosing ellipse (μm2)
|
|---|
| |
HP1β
|
BMI1
|
TRF1
|
|---|
|
Peripheral foci: control
|
0.22 ± 0.12 (n = 10)
|
0.17 ± 0.09 (n = 4)
|
0.19 ± 0.06 (n = 8)
|
|
Peripheral foci: TSA
|
0.13 ± 0.10 (n = 19)
|
0.02 ± 0.02 (n = 12)
|
0.7 ± 0.54 (n = 15)
|
|
Peripheral foci: actinomycin D
|
0.59 ± 0.48 (n = 4)
|
0.09 ± 0.05 (n = 5)
|
0.08 ± 0.05 (n = 20)
|
|
Peripheral foci: vorinostat
|
0.17 ± 0.10 (n = 23)
|
0.17 ± 0.22 (n = 13)
|
0.28 ± 0.47 (n = 29)
|
|
Central foci: control
|
0.15 ± 0.07 (n = 7)
|
0.11 ± 0.05 (n = 10)
|
0.27 ± 0.21 (n = 8)
|
|
Central foci: TSA
|
0.16 ± 0.09 (n = 16)
|
NA (n = 0)
|
0.65 ± 0.42 (n = 8)
|
|
Central foci: actinomycin D
|
0.69 ± 0.46 (n = 6)
|
0.07 ± 0.06 (n = 5)
|
0.05 ± 0.02 (n = 11)
|
|
Central foci: vorinostat
|
0.21 ± 0.12 (n = 13)
|
0.09 ± 0.03 (n = 10)
|
0.15 ± 0.17 (n = 18)
|
|
Area mean: control
|
0.19 ± 0.11
|
0.13 ± 0.07
|
0.23 ± 0.16
|
|
Area mean: TSA
|
0.14 ± 0.09
|
-
|
0.68 ± 0.49
|
|
Area mean: actinomycin D
|
0.65 ± 0.44
|
0.08 ± 0.05
|
0.06 ± 0.04
|
|
Area mean: vorinostat
|
0.18 ± 0.11
|
0.14 ± 0.17
|
0.23 ± 0.39
|
- Mean values ± standard deviations were calculated for all foci found in one living cell. Number of foci analysed is shown in brackets. Central foci were positioned in <60% of nuclear radius, as peripheral foci were considered those located at >60% the nuclear radius. TSA = trichostatin A.
