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Table 1 Area of heterochromatin protein 1β (HP1β), B lymphoma Mo-MLV insertion region 1 (BMI1), and telomeric-repeat binding factor 1 (TRF1) foci

From: Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin

  Area of minimal enclosing ellipse (μm2)
  HP1β BMI1 TRF1
Peripheral foci: control 0.22 ± 0.12 (n = 10) 0.17 ± 0.09 (n = 4) 0.19 ± 0.06 (n = 8)
Peripheral foci: TSA 0.13 ± 0.10 (n = 19) 0.02 ± 0.02 (n = 12) 0.7 ± 0.54 (n = 15)
Peripheral foci: actinomycin D 0.59 ± 0.48 (n = 4) 0.09 ± 0.05 (n = 5) 0.08 ± 0.05 (n = 20)
Peripheral foci: vorinostat 0.17 ± 0.10 (n = 23) 0.17 ± 0.22 (n = 13) 0.28 ± 0.47 (n = 29)
Central foci: control 0.15 ± 0.07 (n = 7) 0.11 ± 0.05 (n = 10) 0.27 ± 0.21 (n = 8)
Central foci: TSA 0.16 ± 0.09 (n = 16) NA (n = 0) 0.65 ± 0.42 (n = 8)
Central foci: actinomycin D 0.69 ± 0.46 (n = 6) 0.07 ± 0.06 (n = 5) 0.05 ± 0.02 (n = 11)
Central foci: vorinostat 0.21 ± 0.12 (n = 13) 0.09 ± 0.03 (n = 10) 0.15 ± 0.17 (n = 18)
Area mean: control 0.19 ± 0.11 0.13 ± 0.07 0.23 ± 0.16
Area mean: TSA 0.14 ± 0.09 - 0.68 ± 0.49
Area mean: actinomycin D 0.65 ± 0.44 0.08 ± 0.05 0.06 ± 0.04
Area mean: vorinostat 0.18 ± 0.11 0.14 ± 0.17 0.23 ± 0.39
  1. Mean values ± standard deviations were calculated for all foci found in one living cell. Number of foci analysed is shown in brackets. Central foci were positioned in <60% of nuclear radius, as peripheral foci were considered those located at >60% the nuclear radius. TSA = trichostatin A.