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Table 1 Area of heterochromatin protein 1β (HP1β), B lymphoma Mo-MLV insertion region 1 (BMI1), and telomeric-repeat binding factor 1 (TRF1) foci

From: Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin

 

Area of minimal enclosing ellipse (μm2)

 

HP1β

BMI1

TRF1

Peripheral foci: control

0.22 ± 0.12 (n = 10)

0.17 ± 0.09 (n = 4)

0.19 ± 0.06 (n = 8)

Peripheral foci: TSA

0.13 ± 0.10 (n = 19)

0.02 ± 0.02 (n = 12)

0.7 ± 0.54 (n = 15)

Peripheral foci: actinomycin D

0.59 ± 0.48 (n = 4)

0.09 ± 0.05 (n = 5)

0.08 ± 0.05 (n = 20)

Peripheral foci: vorinostat

0.17 ± 0.10 (n = 23)

0.17 ± 0.22 (n = 13)

0.28 ± 0.47 (n = 29)

Central foci: control

0.15 ± 0.07 (n = 7)

0.11 ± 0.05 (n = 10)

0.27 ± 0.21 (n = 8)

Central foci: TSA

0.16 ± 0.09 (n = 16)

NA (n = 0)

0.65 ± 0.42 (n = 8)

Central foci: actinomycin D

0.69 ± 0.46 (n = 6)

0.07 ± 0.06 (n = 5)

0.05 ± 0.02 (n = 11)

Central foci: vorinostat

0.21 ± 0.12 (n = 13)

0.09 ± 0.03 (n = 10)

0.15 ± 0.17 (n = 18)

Area mean: control

0.19 ± 0.11

0.13 ± 0.07

0.23 ± 0.16

Area mean: TSA

0.14 ± 0.09

-

0.68 ± 0.49

Area mean: actinomycin D

0.65 ± 0.44

0.08 ± 0.05

0.06 ± 0.04

Area mean: vorinostat

0.18 ± 0.11

0.14 ± 0.17

0.23 ± 0.39

  1. Mean values ± standard deviations were calculated for all foci found in one living cell. Number of foci analysed is shown in brackets. Central foci were positioned in <60% of nuclear radius, as peripheral foci were considered those located at >60% the nuclear radius. TSA = trichostatin A.