Quantitative analysis of polycomb response elements (PREs) at identical genomic locations distinguishes contributions of PRE sequence and genomic environment

Table 2 Primers used in this study.

Primer	Direction	Sequence (5'→3')	Enzyme site
Primers for transgenic constructs
vg1.6 kb	Forward	TAAAGCGGCCGC AAGTCTCCGCCCAATAAT	NotI
vg Δ100	Forward	TAAAGCGGCCGC AGTTTGTGTGAGAGTGAGC	NotI
vg Δ300	Forward	TAAAGCGGCCGC CGTAATTAAAACCGAAGG	NotI
vg 1.6 kb, vg Δ100, vg Δ300	All reverse	GCGCTTTCTA GAGAGCATATAGAAGTGGTCGAA	XbaI
Fab-7	Forward	TAAAGCGGCCGC GGAATTGTGTGGACGATG	NotI
Fab-7	Reverse	GGCGCTTACTAGTGCACAGAGAGTGCAGAAAG'	SpeI
Primers for mutagenesis
vg ΔGAGA	Forward	AAGAGGTTGAACCCCTTGAGGAAACCGGTTTATTTC	-
vg ΔGAGA	Reverse	GAAATAAACCGGTTTCCTCAAGGGGTTCAACCTCTT	-
vg Δ1^stGT	Forward	CAGGAAACCGGTTTATTTCCACAACTCTGTGCGTG	-
vg Δ1^stGT	Reverse	CACGCACAGAGTTGTGGAAATAAACCGGTTTCCTG	-
vg Δ1^st-2^ndGT	Forward	GGTTTATTTCCACAACTCCGTGTGTGCGTTTATGCC	-
vg Δ1^st-2^ndGT	Reverse	GGCATAAACGCACACACGGAGTTGTGGAAATAAACC	-
vg Δ1^st-3^rdGT	Forward	GT TTATTTCCACAACTCCCGTTTATGCCTGTGTGCG	-
vg Δ1^st-3^rdGT	Reverse	CGCACACAGGCATAAACGGGAGTTGTGGAAATAAAC	-
vg Δ1^st-4^thGT	Forward	CAACTCCCGTTTATGCCCGCTAGTTTGTGTGAGA	-
vg Δ1^st-4^thGT	Reverse	TCTCACACAAACTAGCGGGCATAAACGGGAGTTG	-

Bold sequences refer to primer tags that contain a restriction site (named in following column).

ISSN: 1756-8935