Primers for transgenic constructs
| |
vg1.6 kb
|
Forward
|
TAAAGCGGCCGC AAGTCTCCGCCCAATAAT
|
NotI
|
vg Δ100
|
Forward
|
TAAAGCGGCCGC AGTTTGTGTGAGAGTGAGC
|
NotI
|
vg Δ300
|
Forward
|
TAAAGCGGCCGC CGTAATTAAAACCGAAGG
|
NotI
|
vg 1.6 kb, vg Δ100, vg Δ300
|
All reverse
|
GCGCTTTCTA GAGAGCATATAGAAGTGGTCGAA
|
XbaI
|
Fab-7
|
Forward
|
TAAAGCGGCCGC GGAATTGTGTGGACGATG
|
NotI
|
Fab-7
|
Reverse
|
GGCGCTTACTAGTGCACAGAGAGTGCAGAAAG'
|
SpeI
|
Primers for mutagenesis
| | |
vg ΔGAGA
|
Forward
|
AAGAGGTTGAACCCCTTGAGGAAACCGGTTTATTTC
|
-
|
vg ΔGAGA
|
Reverse
|
GAAATAAACCGGTTTCCTCAAGGGGTTCAACCTCTT
|
-
|
vg Δ1stGT
|
Forward
|
CAGGAAACCGGTTTATTTCCACAACTCTGTGCGTG
|
-
|
vg Δ1stGT
|
Reverse
|
CACGCACAGAGTTGTGGAAATAAACCGGTTTCCTG
|
-
|
vg Δ1st-2ndGT
|
Forward
|
GGTTTATTTCCACAACTCCGTGTGTGCGTTTATGCC
|
-
|
vg Δ1st-2ndGT
|
Reverse
|
GGCATAAACGCACACACGGAGTTGTGGAAATAAACC
|
-
|
vg Δ1st-3rdGT
|
Forward
|
GT TTATTTCCACAACTCCCGTTTATGCCTGTGTGCG
|
-
|
vg Δ1st-3rdGT
|
Reverse
|
CGCACACAGGCATAAACGGGAGTTGTGGAAATAAAC
|
-
|
vg Δ1st-4thGT
|
Forward
|
CAACTCCCGTTTATGCCCGCTAGTTTGTGTGAGA
|
-
|
vg Δ1st-4thGT
|
Reverse
|
TCTCACACAAACTAGCGGGCATAAACGGGAGTTG
|
-
|