Figure 5

Mutational analysis of the vg Polycomb response element (PRE). (A) Top: sequence of the first 106 bp of the 1.6 kb vg PRE containing one GAGA site (blue) and four GTGT repeats (red). Bottom: schematic view of deletion constructs for the GAGA site (vg ΔGAGA), the first GTGT repeat (vg Δ1^st GT), the first and second GTGT repeats (vg Δ1^st-2^nd GT), the first to third GTGT repeats (vg Δ1^st-3^rd GT) and the first to fourth GTGT repeats (vg Δ1^st-4^th GT) within the first 106 bp of the 1.6 kb vg PRE are shown. (B) Eye phenotypes of heterozygous and homozygous transgenic vg ΔGAGA and vg ΔGT deletion lines at landing sites (top) 2 and (bottom) 3; 5 day-old male flies are shown. (C,E) Quantification of pigment levels of heterozygous and homozygous transgenic vg ΔGAGA and vg ΔGT deletion lines at landing sites (C) 2 and (E) 3. The vg Δ100 pigment levels from Figure 4 are shown for comparison. (D,F) Pairing-sensitive silencing (PSS) of the transgenic vg ΔGAGA and vg ΔGT deletion lines at landing sites (D) 2 and (F) 3, calculated as in Figure 3.