Reciprocal chromosome conformation capture (3C) scans anchoring at evolutionarily conserved regions. (A) Genomic organization of the Kcnq1 region scanned in this study, spanning 387 kb. Dark boxes in the Kcnq1 gene are exons. Arrows show direction of transcription; maternal-specific and paternal-specific expression is indicated above and below the line. Reactivated Kcnq1 expression is depicted as a red arrow. Vertical bars indicate the Afl III/Nco I restriction sites analyzed in this study, with the fragments assayed with the variable primers for contact with the tissue-specific evolutionarily conserved regions (ECRs) numbered. White arrowhead, anchor primer for neonatal heart; gray arrowhead, anchor primer for neonatal brain. Graphical representation is not to scale. (B) Relative crosslinking frequency of regions interacting with ECR 4. The peaks correspond to the Kcnq1 promoter and fragment 12 within intron 1. (C) Relative crosslinking frequency of regions interacting with ECR 2. The peaks correspond to the Kcnq1 promoter and fragment 12 within intron 1. Hatched vertical lines represent the anchor position.