Myc represses expression of differentiation-associated including homeobox ( Hox ) genes, a function antagonized by Miz-1. (A) Confirmation of gene expression changes in c-myc knockdown (KD) embryonic stem (ES) cells, determined by gene expression array, by quantitative real-time (qRT)-PCR PCR. Real-time qRT-PCR of a series of differentiation-associated genes or metabolism related genes. Error bars are standard deviations. N = 3. (B) Venn diagram showing overlap among differentially expressed genes in Myc KD or Miz-1 KD ES cells. Gene ontology (GO) analysis of overlapping genes presented below the Venn diagram. P values are indicated.