Genomic binding of Myc and functional ontology of its target genes in human embryonic stem (ES) cells. (A) Acetylated lysine 9 and trimethylated lysine 4 of histone H3 (AcH3K9 and H3K4me3) status for Myc bound genes. Gene ontology analysis for Myc bound genes with or without enrichment in histone activation marks H3K4me3 and AcH3K9 was performed using DAVID functional annotation software. P values are indicated. Myc binding events that do not overlap with either histone modification are represented by the blue circle. (B) Confirmation of Myc direct targets identified by chromatin immunoprecipitation/microarray (ChIP-chip). ChIP was performed using antibodies to c-Myc, N-Myc, AcH3K9, and H3K4me3. PR2Y2 gene, not bound by either c-Myc or N-Myc, was used as a negative control.