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Figure 4 | Epigenetics & Chromatin

Figure 4

From: Proliferating cell nuclear antigen is required for loading of the SMCX/KMD5C histone demethylase onto chromatin

Figure 4

Association of SMCX (Smcy homolog, X-linked (mouse)) and proliferating cell nuclear antigen (PCNA). 293T cells were transfected with the SMCX-encoding plasmid, and chromatin lysates prepared 2 days after transfection. Lysates were then immunoprecipitated with anti-SMCX, anti-PCNA, or control normal rabbit or mouse IgG, respectively. Some lysates were pretreated with DNAse, as described in Methods. SMCX and PCNA were detected by western blotting. D = DNAse-pretreated samples, immunnoprecipitated with the corresponding antibody, Ig = control normal IgG (mouse for the anti-PCNA antibody (top), and rabbit for the anti-SMCX antibody (bottom)), In = input lysate, P = samples immunoprecipitated with the anti-PCNA antibody, S = samples immunoprecpitated with the anti-SMCX antibody.

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Epigenetics & Chromatin

ISSN: 1756-8935