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Figure 1 | Epigenetics & Chromatin

Figure 1

From: Proliferating cell nuclear antigen is required for loading of the SMCX/KMD5C histone demethylase onto chromatin

Figure 1

Effect of proliferating cell nuclear antigen (PCNA) knockdown on the intracellular distribution of exogenous SMCX (Smcy homolog, X-linked (mouse)) protein. 293T cells were transfected with the SMCX-encoding plasmid or the control empty vector, and PCNA or control small interfering (si)RNA. Two days after transfection, cells were harvested, then the chromatin fraction was separated from the rest of the lysate and the fractions analyzed by western blotting. The four samples on the left are of SMCX and PCNA levels in chromatin, and those on the right are SMCX levels in the whole-cell lysate without chromatin fraction. Histone H3 (chromatin protein) and TRim5α (cytoplasmic protein), respectively, were used as loading controls. The label 'Vector' indicates cells transfected with the empty plasmid vector.

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Epigenetics & Chromatin

ISSN: 1756-8935