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Figure 5 | Epigenetics & Chromatin

Figure 5

From: Pluripotency factor binding and Tsix expression act synergistically to repress Xist in undifferentiated embryonic stem cells

Figure 5

Analysis of Xist expression in lipofected cell pools. (A) Southern blot analysis of DNA extracted from the cell pools 12 days after lipofection of P1 transgenes into undifferentiated XY ES cells. Data for two experiments are shown. (B) qRT-PCR analysis of Xist expression in XY ES cell pools transfected with P1 transgenes. All data is normalised to β-actin and wt Xist transcript level in XY ES cells (129/1). The average value from three independent experiments (+ SEM) is shown for each transgenic genotype. ES: embryonic stem; Msx: homeobox, msh-like 1 autosomal gene used for internal normalisation; qRT-PCR: quantitative reverse transcription polymerase chain reaction; R: Raoul marker; wt: wild type.

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