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Figure 8 | Epigenetics & Chromatin

Figure 8

From: Chromatin regulated interchange between polycomb repressive complex 2 (PRC2)-Ezh2 and PRC2-Ezh1 complexes controls myogenin activation in skeletal muscle cells

Figure 8

Myogenin ( MyoG ) transcriptional activation, via recruitment of MyoD, is regulated by the PRC2-Ezh1 complex. Chromatin immunoprecipitation (ChIP) analysis was performed using chromatin prepared from C2C12 cultured in growth medium (GM) or differentiation medium (DM) (24 h from the induction of differentiation, the point at which MyoG is activated), after treatment with Ezh1 (oligo no. 2) and Ezh2 (oligo no. 2) small interfering RNA (siRNA), using MyoD antibody at the MyoG promoter and muscle creatine kinase (mCK) enhancer in (A) and RNA polymerase II (RNA Pol II) and H3K4me3 antibodies at different regulatory regions of MyoG gene in (B), as represented at the top of this panel. The precipitated DNA fragments were subjected to real-time PCR. Levels of H3K4me3 were normalised to histone H3 density. The values, shown as percentage of the input, represent the mean of three independent experiments. The mock was less than 0.01%. Error bars represent standard deviation.

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Epigenetics & Chromatin

ISSN: 1756-8935